Betanin, besides displaying an inherent colorant capacity, was equally effective as a natural antioxidant displaying peroxy-radical scavenger ability in pork meat. Betanin high antioxidant ability was retained even after simulated small intestine digestion. Half of the betanin amount was recovered in the small intestine digestive fluid and no traces were found after colon fermentation. The stability and antioxidant ability of this compound were assessed during a human digestion simulation and ex vivo colon fermentation. Betanin showed significant stability up to −30 ☌ and mild stability at chilling temperature. Herein, betanin was purified by semi-preparative HPLC-LC/MS and identified by LC-ESI(+)-MS/MS as the pseudomolecular ion m/ z 551.16. However, the antioxidant power and health-promoting properties of this pigment have been disregarded, perhaps due to the difficulty in obtaining a stable chemical compound, which impairs its absorption and metabolism evaluation.
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